Structural insights and biomedical potential of IgNAR scaffolds from sharks

paper8Published online on 14. January 2015

Structural insights and biomedical potential of IgNAR scaffolds from sharks

Stefan Zielonka, Martin Empting, Julius Grzeschik, Doreen K├Ânning, Caroline J Barelle, Harald Kolmar

ABSTRACT:

In addition to antibodies with the classical composition of heavy and light chains, the adaptive immune repertoire of sharks also includes a heavy-chain only isotype, where antigen binding is mediated exclusively by a small and highly stable domain, referred to as vNAR. In recent years, due to their high affinity and specificity combined with their small size, high physicochemical stability and low-cost of production, vNAR fragments have evolved as promising target-binding scaffolds that can be tailor-made for applications in medicine and biotechnology. This review highlights the structural features of vNAR molecules, addresses aspects of their generation using immunization or in vitro high throughput screening methods and provides examples of therapeutic, diagnostic and other biotechnological applications.

mAbs, Volume 7, Issue 1, doi 10.4161/19420862.2015.989032

SOURCE ( OPEN ACCESS )

 

 

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